How to reconstitute peptides

Reconstituting means adding bacteriostatic water to a vial of freeze-dried powder so it can be measured. The steps below describe common research handling.

Steps

  1. Gather your supplies. You need the peptide vial, bacteriostatic water, alcohol swabs and a syringe to measure the water.
  2. Clean both stoppers. Wipe the rubber tops of the peptide vial and the water vial with an alcohol swab and let them dry.
  3. Draw the water. Draw the amount of bacteriostatic water you planned, for example 2 mL.
  4. Add it slowly down the side. Push the needle through the stopper and let the water run down the inside wall of the vial instead of onto the powder.
  5. Swirl, do not shake. Roll or gently swirl the vial until the powder dissolves. Shaking can damage the peptide.
  6. Label and refrigerate. Write the date and the amount of water on the vial and store it in the fridge.
  7. Work out the units. Divide the vial mg by the water mL to get concentration, then use the calculator to find the syringe mark for a given amount.

How much water to add

There is no single right amount. Less water makes the solution stronger, so each measurement is fewer units. More water makes small amounts easier to measure. A common target is a draw of at least a few units, which the BAC water optimizer can solve for.